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A new series of rII mutations in the virulent phage T4 has been isolated, and th

ID: 30640 • Letter: A

Question

A new series of rII mutations in the virulent phage T4 has been isolated, and

the results of phage recombination experiments between all twelve of the

new mutations are listed below:

Draw a inear genetic map showing the location of all point mutations and

the frequency of recombination between adjacent mutations. Describe the

location and extent of all deletions.

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1 234 56 7 8 910 11 12 1 0 0.2 3.5 0.5 2.8 1.5 2.0 1.9 0.7 1.0 0.53.0 0 3.70.7 3.0 1.72.22.1 0.9 1.20.3 3.2 0 3.0 0 2.0 1.5 1.61.4 2.5 4.00.5 0 2.3 1.0 1.5 1.4 0.2 0.5 1.0 2.5 2 4 0 1.3 0.8 0.9 0.7 1.8 3.3 0 0 0.5 0.4 0 0.5 2.0 1.5 0 0.1 0 1.0 2.5 1.0 0 0 0.9 2.4 1.1 0 0 1.2 0.9 0 1.5 2.0 0 3.5 6 7 9 10 12 0

Explanation / Answer

Understanding pathogen infectivity and virulence requires combining insights from epidemiology, ecology, evolution and genetics. Although theoretical work in these fields has identified population structure as important for pathogen life-history evolution, experimental tests are scarce. Here, we explore the impact of population structure on life-history evolution in phage T4, a viral pathogen of Escherichia coli. The host

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