you are researching a disorder that arises from a mutation in the gene x. the di
ID: 303441 • Letter: Y
Question
you are researching a disorder that arises from a mutation in the gene x. the disease causing mutation occurs at amino acid 43. using primers specific to the region of dna that codes for amino acids 38 to 43 (15 bp long) you complete a pcr on a patient sample that you suspect possesses this mutation. you then perform sanger sequencing on the pcr product.
a) what is the purpose of PCR prior to sanger sequencing?
b) the native (unmutated sequencing) of amino acids 38-40 of examase is : Arg-Thr-*ARG*-Gln-Asp
Explanation / Answer
a. The Polymerase chain reaction or PCR is performed before Sanger sequencing in order to have the multiple copies of target fragment that need to sequenced with the help of specific DNA primer.
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