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Background and/or Instructions Problem N\" 10 For a certain type of animal tissu

ID: 303399 • Letter: B

Question

Background and/or Instructions Problem N" 10 For a certain type of animal tissue, the probability of a randomly selected cell being in a particular stage of the interphase/mitosis cycle is given by the following table: Objective: To review the phases of mitosis with the understanding that in a real tissue or a non-synchronized cell culture different mitotic phases and cells in interphase will be observed at a certain time. Stage terphase Prophase Metaphase Anaphasee Telophase Probability 0.5 0.1 0.05 0.2 0.15 Interphase is the period between mitotic events; all other phases are part of mitosis and their individual probabilities add up to the total probability of seeing cells undergoing mitosis. Pay attention to the individual probabilities for cells in each phase of the cell cycle Suppose you examine 100 cells at random from this tissue. A) How many cells do you expect to see in metaphase?cells B) You have selected a high magnification (2000 X) in your microscope and you see only one cell at a time (50 um in diameter). How frequently you would see a cell undergoing mitosis?- % c) what is the probability of seeing no metaphases in 100 cells? %

Explanation / Answer

Answer a. Probability of metaphase is 0.05 out of 100 cells.

It can be calculated as:

Probability = M/total cells

0.05 = M/100

Means, M= 100 X 0.05 = 5 cells in metaphase out of 100 cells.

Answer b. 50%

Because 50% cells are in Interphase stage, which occurs prior to the mitosis. In Interphase, the cell prepares itself for mitosis. As per adding the probability value of prophase, metaphase, anaphase and telophase the number of cells comes 10,5,20 and 15 respectively. Adding these values makes 50.

Answer c. Only 5 cells out of 100 cells are in metaphase. So, the other 95 cells are not in metaphase.

Probability of not in metaphase is= 95/100 = 0.95