Attached is a simplified network and data on the composition of Escherichia coli
ID: 3030435 • Letter: A
Question
Attached is a simplified network and data on the composition of Escherichia coli. The information can be used to develop a solution for the rates (r) Ar-b where A represents the stoichiometric information from metabolism (0, 1, -1, and a single 2 at the point where one transitions from a C6 to a C3 compound) and b represents the biosynthetic demand, network relationship (zero or non-zero value), or measurement (glucose uptake). An example of a stoichiometric relationship would be the balance around glucose 6 phosphate r(1) (2) r(3) r(10) if r(1) rate of glucose uptake, r(3) was the amount of glucose 6 phosphate shunted off to make the cell wall, r(2) was the amount of glucose 6 phosphate used to make ribulose 5 phosphate, and r(10) was the amount of glucose 6 phosphate used to make fructose 6 phosphate. An example of a measurement would be the uptake of glucose by the cell: if I measured the amount of glucose via GC or HPLC. Note that the first term in parenthesis is a conversion factor and the last is the dry cell weight (x, DCW) and the middle term is the specific growth rate of the cells. Finally, an example of a biosynthetic demand would be r(27) 1.078 mmol/g cell (0.2 h if I used the data in the accompanying table to determine how much a-ketogluterate ended up as amino acids and nucleic acids. All values of r(i) and b (i) have the units of mmol/g cell h, which looks similar to a reaction rate with a different basis (mmol/l h) You are to calculate the rates r(1) through r(32). Your answer is to be structured as follows de 1 fiinrtinn that stoichiometric matrix A. Organize your matrix in a builds the 32x32Explanation / Answer
Following is the program to find the count of zeros in 32 line A=32x32 matrix B=zeros(32) for i= 1:32 for j=1:32 B(i,j)=A(i,j) end end
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