1o. il color Is o iy developed aitel,ncbut ut u a longer period of time. Describ

Question

1o. il color Is o iy developed aitel,ncbut ut u a longer period of time. Describe the mechanism of ELISA. Why is ELISA so sensitive? Why is it necessary to block unoccupied binding sites in the mi- crotiter wells? Why is it important to have a positive control? 1. Why can the onset of AIDS take several years? 2. 3. 4. Why is anti-HIV-1 IgG screened instead of the virus itself? Why does the destruction of TH cells compromise the entire im- mune system? How does HIV target T, cells? 5. Why are there so many immunological variants of HIV? The elimination of several steps in the ELISA could be accomplished if the primary antibody was mmade into an enzyme conjugate. Why is this generally not done? What can cause a false positive in an ELISA? 6. use of accomp distributed for ar ved.

Explanation / Answer

Part-1- ELISA precept foundation and extension. Enzyme-connected immunosorbent assays (ELISA) combine the specificity of antibodies with the sensitivity of simple enzyme assays, through using antibodies or antigens coupled to an effortlessly-assayed enzyme. ELISA can provide a beneficial measurement of antigen or antibody concentration.ELISA precept foundation and extension. Enzyme-related immunosorbent assays (ELISA) combine the specificity of antibodies with the sensitivity of easy enzyme assays, by the use of antibodies or antigens coupled to an without difficulty-assayed enzyme. ELISA can provide a useful dimension of antigen or antibody attention.

ELISA is so sensitive because of the detection method, i.e. the use of antibody, and visual detection, a POSITIVE CONTROL is needed because of the relative selectivity of the antibody. It may continually bind to other stuff and supply artifactually high values. It is necessary to remove unbound site from microtiter plate so that they can not effect the test result while reading in ELISA plate reader.

Part-2- It could remain in a latent provirus recombinant until activation and it also slowly kills wbcs.

Part-3- for motives of protection, stability, and reproducibility.

Part-4- The immune system is weakened at this point and macrophages are incapacitated by the helper t cells state of no activity. By means of infecting other cells of the immune device first and understanding that helper t cells will come to that site.

Part-5- This is because of high mutation rate.

Part-6- Making an antibody-enzyme conjugate isn't trivial because the usage of a number one/secondary set-up you may use the same well-characterized conjugate in combination with many distinctive number one antibodies (as long as those primaries are all raised in the equal species). There may be additionally the opportunity of a few amplification: for instance, if the secondary is an anti-fab then two secondary igs will bind to every number one and false can be cause by not removal of unbound antibody and antigen thorugh washing.

Related Questions

Navigate

• Browse (All)
• Browse 1
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.