Write your conclusion about the effect of pH on lactase reaction rate. Do the da
ID: 302684 • Letter: W
Question
Write your conclusion about the effect of pH on lactase reaction rate. Do the data support your prediction? If so, explain how the data supports your prediction. If not, explain what you think may have occured. What is the optimum pH for lactase (based on your data)?
Write your conclusion about the effect of temperature on lactase reaction rate. Do the data support your prediction? If so, explain how the data supports your prediction. If not, explain what you think may have occured. What is the optimum pH for lactase (based on your data)?
Write your conclusion about the effect of substrate concentration on lactase reaction rate. Do the data support your prediction? If so, explain how the data supports your prediction. If not, explain what you think may have occured. What is the optimum pH for lactase (based on your data)?
Absorbance at 450nm (A450) pH7 0 0.068 0.14 0.213 0.265 pH10 0 0.052 0.115 pH12 0 0.001 0.002 0.003 0.004 Time(min) 0 0.5 pH2 0 0.002 0.004 0.005 0.006 pH4 0 0.6 0.129 0.194 0.265 1.5 0.24Explanation / Answer
Effect of pH
Lactase is an enzyme that catalyzes the breakdown of lactose into one molecule of glucose and galactose. optimum pH for lactase activity is 7 but can also function in acidic and basic medium from 2 to 8, deviation from this range cause denaturation of lactase enzyme, from table 1 it has been shown that deviation in pH usually below 2 and above 10 slows rate of reaction by the decrease their absorbance
Effect of temperature
, the optimum temperature for its function ranges approximately 70 and 120 degrees Fahrenheit or approximately 37c o, when temperature goes below their optimum range alters the rate of lactase's function, while temperature goes above 50Co or over 135 degrees Fahrenheit, lactase became denatured and losses their activity, as shown in table 2, absorbance from 25 to 50 C increase with time but variation in optimum temperature shows deviation in absorbance as shown in Oco and 100Co
Substrate concentration
the rate of reaction of an enzyme is directly proportional to the concentration of substrates, from table 3
it has been clearly shown that the rate of absorbance increases with an increase in substrate concentration
optimum pH 7
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