7. The addition of mineral oil to the top of the Arginine Decarboxylase (ADC) me

Question

7. The addition of mineral oil to the top of the Arginine Decarboxylase (ADC) medium promotes fermentation of the glucose in the medium by creating an anaerobic environment. If glucose is fermented, this lowers the pH of the medium and it turns yellow. The enzyme Arginine Decarboxylase is activated at low pH, and the catalytic activity of this enzyme causes the pH to rise, turning the medium purple. A. If you forgot to add mineral oil to the top of the tube, is this more likely to produce a false positive or a false negative? false Negative B. You may have noticed that our mineral oil is in dropper bottles which are shared by the whole lab. These bottles are definitely not sterile. Is using these dropper bottles to put mineral oil on the top of ADC medium more likely to produce a false positive or false negative? fnlse Postive

Explanation / Answer

7) A) If you forgot to add mineral oil on the top of the tube, it is more likely to produce false negative.

B) By using these dropper bottles to put mineral oil on the top of ADC medium, it is more likely to produce false positive.

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