Escherichia coli Pseudomonas malli Bacillus megaterium Bacillus anthracis , Lact
ID: 302319 • Letter: E
Question
Escherichia coli
Pseudomonas malli
Bacillus megaterium
Bacillusanthracis,
Lactobacillusacidophilus,
Pseudomonas aeruginosa,
Serratia marcescens
Staphylococcus caprae
Streptococcus mutans
Neisseria gonorrhoeae,
Staphylococcus aureus,
Streptococcus pyogenes,
Proteus myxofaciens,
Klebsiella pneumoniae sub sp pneumoniae
Salmonella bongrori
Shigella sonnei
Corynebacterium xerosis
Enterobacter aerogenes
Proteus vulgaris
Escherichia vulneris
Escherichia blattae
Salmonella choleraesuis (subsp. diarizonae)
Enterobacter hormaechei
Enterobacter asburiae
Citrobacter diversus
Citrobacter freudii
In order to receive full credit for this assignment, you must provide the following;
The correct identification of Unknown organism in this homework assignment.
The correct interpretation of the biochemical tests in the table below for this unknown organism.
Create a flow chart which demonstrates the tests that allowed you separate the unknown organism from the list.
Use Microsoft PowerPoint, it is easier for creating flow charts compared to Microsoft Word
You do not need to isolate the entire list of organisms from each other, just your unknown from the list. See the sample flow chart.
Using your biochemical and morphological results to indentify the identity of your orphan organism by using the Bergey’s manual.
Create a flow chart and identify your orphan organism.
UNKNOWN # 3
The follow biochemical tests were performed after an unknown bacterium was isolated into a pure culture. Each of the tests was incubated at 37°C for 24 hours and when appropriate the use of reagents was added in the correct volumes to each of the tests. The following table describes these results.
Name of the Biochemical Test/ Media
Appearance after addition of the reagent
POS/NEG
Interpret the Resulting Reactions of the test
( POS or NEG) 1 pts each
Catalase (example)
Bubbles
POS
POS for catalase/ H2O2 àH20 + O2
Urea/ Urea broth
Reddish / yellow
Phenol red Lactose
Red
Phenol red Mannitol
Yellow
Simmons Citrate
Blue
Methyl red (MR)/ MRVP broth
Red
Vogus Proskauer (VP)/MRVP broth
No change/copper color
Indole production
/ SIM media
No red ring
Phenol red Glucose
Yellow
Gram Stain
Pink
FTM tube/ fluid thioglycollate media
Growth though out the tube
0.4% Agar with Nutrient broth and1% TTC.
Growth and Red though out the tube
Starch Hydrolysis/ Starch agar
Brown around the inoculation
Gelatin Hydrolysis/ Gelatin media
Solid
Oxidase
No color change
Nitrate reduction/ Nitrate broth
Red After Nitrate A&B reagent is added
Shape
ROD
Fat hydrolysis/ Spirit blue agar
White around the inoculation
H2S production/ SIM media
Black
Name of the Biochemical Test/ Media
Appearance after addition of the reagent
POS/NEG
Interpret the Resulting Reactions of the test
( POS or NEG) 1 pts each
Catalase (example)
Bubbles
POS
POS for catalase/ H2O2 àH20 + O2
Urea/ Urea broth
Reddish / yellow
Phenol red Lactose
Red
Phenol red Mannitol
Yellow
Simmons Citrate
Blue
Methyl red (MR)/ MRVP broth
Red
Vogus Proskauer (VP)/MRVP broth
No change/copper color
Indole production
/ SIM media
No red ring
Phenol red Glucose
Yellow
Gram Stain
Pink
FTM tube/ fluid thioglycollate media
Growth though out the tube
0.4% Agar with Nutrient broth and1% TTC.
Growth and Red though out the tube
Starch Hydrolysis/ Starch agar
Brown around the inoculation
Gelatin Hydrolysis/ Gelatin media
Solid
Oxidase
No color change
Nitrate reduction/ Nitrate broth
Red After Nitrate A&B reagent is added
Shape
ROD
Fat hydrolysis/ Spirit blue agar
White around the inoculation
H2S production/ SIM media
Black
Explanation / Answer
Urea - Reddish yellow - negative
Phenol red - Red - negative
Phenol red mannitol - positive - change in pH of the medium
Simmons citrate - Blue - positive - change in pH causes color change
Methyl red - Red - positive - mixed acid fermnetation
Vogus proskauer - no change - negative - acetyl methyl carbinol is absent
Indole - No ring - negative - no indole production
Phenol red glucose test - Yellow - positive - fermentation , changespH
gram stain - pink - gram negative
FTM tube - throughout growth -facultatively anerobe
0.4% agar ... (motility test ) - Non motile
starch hydrolysis - positive - amylase production
Gelatin hydrolysis - negative - no gelatinase production
Oxidase - negative - no oxidase production
Shape - Rod
Fat hydrolysis - positive - hydrolysis of fat
H2s - black precipitate - positive - H2s production.
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