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1. Which of the following is/are required for the polymerization reaction cataly

ID: 302296 • Letter: 1

Question

1. Which of the following is/are required for the polymerization reaction catalyzed by RNA polymerase?

1) RNA primer

2) DNA primer

3) RNA template

4) DNA template

5) NTPs

6) dNTPs

7) Magnesium

8) Calcium

A) 4, 5, and 6

B) 1, 3, 5, and 7

C) 1, 3, 5, and 8

D) 4, 5, and 7

2. RNA polymerase is best described as a ________.

A) DNA-dependent DNA polymerase

B) RNA-dependent DNA polymerase

C) RNA-dependent RNA polymerase

D) DNA-dependent RNA polymerase

3. Eukaryotic RNA polymerase II requires which proteins for selective binding?

A) ? subunit

B) ? subunit

C) Transcription factors

D) ? subunit

4. A deficiency in intracellular rRNA can be attributed to which eukaryotic enzyme?

A) RNA polymerase II

B) DNA polmerase I

C) RNA polymerase III

D) RNA polymerase I

Explanation / Answer

1.

D) 4, 5, and 7

RNA polymerase uses DNA template to polymerise NTPs to form RNA. Magnesium ions are required at the active sites of RNA polymerase.

2.

D) DNA-dependent RNA polymerase

RNA polymerase uses DNA as template to synthesise RNA.

3.

C) Transcription factors

4.

D) RNA polymerase I

RNA polymerase II forms mRNA, RNA polymerase III forms tRNA as well as other RNAa including a few rRNAs, and RNA polymerase I forms rRNA.