1:53 AM @ * 10%. Study .. : ? Meme Watch Queen Sugar Online Quizzes-Blochemistry

Question

1:53 AM @ * 10%. Study .. : ? Meme Watch Queen Sugar Online Quizzes-Blochemistry SecC Home/Ch Tube frame anou-1555589&isprvIdrc.; 1 &q;= 191 mistry Section 131 Summer 2018 CO sessments Com Course ResourcesCourse Ho 3 Take Home (Ch 12 14 15 16 17 18 19) ana Banks: Attempt 1 Question 77 (1 point) ? a FRET experiment was carried out with bare fluorescent protein attached to the a dont of a stimulatory Gprotein and a green fluorescent protein attached to the ? subunit, the presence of a strong FRET signal would be associated with al interaction of a G protein-coupled receptor with an antagonist hydrolysis of GTP in the ? suburit s of cAMP both Interactioh of a G protein-coupled receptor with an antasonist and hydrolysis of GTP in the o subunit e) both trydroty of CTP in the ? subunit and increased concentrations ofcAMR Question 78 1

Explanation / Answer

Answer: The Fluorescence resonance energy transfer (FRET) takes place when a donor fluorophore in excited state transfers its excitation energy to a nearby chromophore, the acceptor. Blue fluorophore is a donor and green fluorophore is acceptor. The excitation takes place when the protein tagged with fluorophore undergo conformational changes. Since G-protein undergo conformational changes when it binds to a ligand. Consequently, strong signal flash will be assocoated with the donor fluorophore that is when G-protein coupled receptor binds with ligand.

Option (1) is TRUE.

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