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Write the technique with on application DNA fingerprinting_ analyzing trace amou

ID: 3010996 • Letter: W

Question

Write the technique with on application DNA fingerprinting_ analyzing trace amounts of degraded, ancient DNA___ making a cDNA library__ identifying a gene in genomic DNA. if sequence is unknown, but a probe is available_ Write the technique with on application identifying sequences complementary to a probe___ separation of large DNA molecules__ Introducing recombinant DNA into host cells__ using synthetic oligonucleotide primers to direct the PCR amplification of a DNA fragment__ The restriction enzyme EcoRI cuts a linear 19 kb piece of DNA into fragments of 1, 3, 6, and 9 kb. How many EcoRI sites arc there in the 19 kb piece of DNA? How many fragments can be obtained for the same DNA circular?

Explanation / Answer

8.

a)

DNA fingerprinting is a test to identify and evaluate the genetic information—calledDNA (deoxyribonucleic acid)—in a person's cells

DNA fingerprinting is done to:

b)

Trace DNA analysis: forensics

c)

making a cdna library: Plant and soil sciences.

d)

Very large DNA molecules were separated by electrophoresis in horizontal slab gels of dilute agarose