MIcrobio lab final _____________ enzyme reduces Nitrate to nitrite. Reduction is
ID: 300869 • Letter: M
Question
MIcrobio lab final
_____________ enzyme reduces Nitrate to nitrite.
Reduction is ____________ (loss or gain) of electrons
In Nitrate reductions test- the source of nitrate is ________ (bacteria or medium)
________ compound reduces nitrate to nitrite
What is the chemical name of Nitrate I Reagent? ____________ _ _______________
What is the chemical name of Nitrate II Reagent? ____________ _____________
Explain (in 2-3 sentences) why the nitrate medium changes to red color after adding Nitrate reagent I and II to some cultures?
Explain (in 2-3 sentences) why the nitrate medium changes turns clear after zinc to some cultures?
The differential agent phenylalanine in Phenylalanine Deaminase test is _______________ (Sugar, or amino acid or protein)
In the Phenylalanine Deaminase test the green color formed is due to the reaction between ____________ and ________________
In BESC test the selective agent ___________ inhibits all Gram + bacteria except ____________
In BESC test the differential agent ___________ is hydrolyzed to ___________
In BESC test the indicator ___________ reacts with _____________ to form dark color
The gas produced in KIA tube is due to A or B by bacteria.
A) sulfur reduction to H2S B)fermentation of sugars
The black color change in KIA tube is due to A or B by bacteria.
A) sulfur reduction to H2S B)fermentation of sugars
Describe how you will test and identify the susceptibility of bacteria to a specific antibiotic
What is the other name for ‘Antimicrobial Susceptibility Test’?
Describe briefly the steps in the above test?
Mention at least 4 common antibiotic discs used in the above test.
Give one example where the ‘Antimicrobial Susceptibility Test’ is used.
In Lysine decarboxylase test mineral oil is added after inoculation to create ___________environment
IMViC test purpose is to differentiate ____________ from ______________
Expand IMViC__________________________________________.
To MRVP broth what reagents are added for testing
the production of mixed acids by fermentation _____________
the production of alcohol or acetoin ___________________________
Phenol Red media test for the ability of bacteria to metabolize ____________ sugars to ___________.
Phenol Red media test tubes contain ___________ to test for the ability of bacteria to produce gas by fermenting sugars.
Name two sugars used in Phenol red media: ____________ and ___________
In SCA media the positive test shows __________ color change from __________ color.
In Simmons citrate test release of ___________ increases the pH of the media
Bacteria that contain _______________________ enzyme (removes carboxyl) will be able to metabolize lysine amino acid.
In urea hydrolysis test the _________ color is positive for urea test
Some bacteria can breakdown gelatin with the help of __________ enzyme they produce.
or
Some bacteria carry out gelatin _____________ reaction which is catalyzed by gelatinase enzyme which turns the media from _____ (state) to __________ (state)
The gelatin media inoculated should be incubated at 25? C.
Name the media used for identifying organisms that are motile by showing cloudiness throughout the tube
A) SCA B)DNA media C) SIM D)MRVP
or
In SIM media _________ indicates the presence of motile bacteria.
____________ radiation causes thymine dimers
A. Infrared B. Visible light C. UV D. Microwaves
DNA is damaged in bacteria exposed to harmful radiation but they recover by _____ DNA repair enzymes that require light
DNA is damaged in bacteria exposed to harmful radiation but they recover by _____ DNA repair enzymes that do NOT require light
A. Exonucleases B. Endonucleases
C. Photolyases D. ligases
UV rays _______ pass through plastic and glass
A. Easily B. Poorly C. do not
Transformation is the ability of bacteria to take up DNA from environmental phage. A. True B. False
___________ and ___________ are required to make E. coli cells competent to take up DNA
A. CaCl2 transformation solution and heat shock
B. LB broth and plasmid DNA
C. ice bath and water bath
___________ gene is used for selecting the transformants.
A. Arabinose promoter B. Ampicillin resistance
C. pGLO plasmid D. GFP
___________ are small, naturally occurring, circular DNA molecules that possess few genes and ORI
ChromosomesB. PlasmidsC. Genes
GFP gene in the plasmid will be transcribed and translated in
A. LB/amp plate +DNA B. LB/amp/ara plate +DNA
C. LB/amp plate –DNA D. LB plate–DNA
“LB plate –DNA” allows growth of E.coli colonies which were not transformed.
A. True B. False
ELISA stands for _____
List 2 advantages and one disadvantage of ELISA
Results: Slide agglutinations shows ________ whereas ELISA shows_________ ______
______ test is a simple procedure that is used to detect susceptibility to dental decay
In _____ test, color change is an indicative of presence of dental- decay causing bacteria and _______ was used for sample.
_______ sugar is used to favor the growth of oral bacteria
E__________ is the study of diseases
Determining the ______ _____ is the main objective of E______ simulation lab activity.
Largest white blood cell (WBC) ____________
WBC with 4-5 lobes ____________
WBCs which are found in large numbers in normal blood sample _____________
Presence of 6 Eosinophils out of 200 total WBCs counted is an indication of ____________.
In a patient sample Number of Monocytes were 14%. What does this suggest
Name the agranulocytes _____________ and granulocytes __________________
Explanation / Answer
Please ask questions separately and not all at once
Nitrate reductase
Gain of electrons
medium(this test checks the ability of the microbe to reduce nitrate from medium to nitrite )
Sulfanilic acid
N ,N-dimethylnaphthylamine
This test is based on the detection of nitrite in the medium formed by reduction of nitrate by microbe that is present. If present in the medium, nitrite will react with sulfanilic acid (Nitrate reagent A) to form a colorless complex (nitrite-sulfanilic acid). This complex forms a red precipitate (prontosil) with nitrate reagent B (alpha-naphthylamine).
Some strains may cause reduction of the nitrate to nitrite, and then to nitrogenous gases which are not detected when Nitrate reagents A and B are added to the medium. The zinc dust is a catalyst and catalyses the reduction of nitrate to nitritenitrite .If no color develops in the medium after the addition of zinc dust, the micorganisms may have reduced nitrite to nitrogenous gases; thus being nitrate-positive.
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