standardized genetic parts and restriction enzymes. This standardization allows

Question

standardized genetic parts and restriction enzymes. This standardization allows parts of genes to be used interchangeably, and for the easy assembly of large genetic constructs. You can find information about the most common assembly standard used in iGEM (RFC 10) here: http://parts.igem.org/Assembly Standard assembly https://www.youtube.com/watch?v 5Cr5YkvN8YO 1. What restriction enzymes produced the two sequences below? If the sequences below are hybridized and ligated, explain why it would not be possible to cut the new sequence with either of the original restriction enzymes. a) 5'-T 3'-AGATC b) CTAGT-3' A-5'

Explanation / Answer

Resctrion enzymes cleaves the DNA at specific sites which are called resctrion sites.The sequence in these sites are specific

A) is Xbal 5' TCTAGA3'

3' AGATCT5'

B) is Spel 5' ACTAGT3'

3'TGATCA5'

Xbal + Spel = 5' TCTAGT3'

3' AGATCA5'

BECAUSE The ability of the enzymes to cut DNA at precise locations disabled researchers to isolate gene-containing fragments

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