QUESTION 7 (2 marks) You are given a plasmid containing a gene from Drosophila m
ID: 300520 • Letter: Q
Question
QUESTION 7
(2 marks)
You are given a plasmid containing a gene from Drosophila melanogaster. The gene is 303 base-pairs long.
You would like to amplify it using PCR, therefore, you design oligonucleotide primers 19 nucleotides in length that are complementary to the plasmid sequences immediately adjacent to both ends of the cloning site.
What would be the exact length of most of the resulting PCR products (in base pairs)?
The PCR product would be base pairs in length.
2 points
QUESTION 8
(3 marks)
Which two primers would result in an amplified PCR product?
The primers Primer 1 Primer 2 Primer 3 and Primer 1Primer 2Primer 3 would result in an amplified PCR product.
HINT: Synthesis of DNA ALWAYS proceeds 5’ to 3’.
Explanation / Answer
Answer of Question 7
The exact length of the amplified piece of DNA will be of 341 base pairs
19 + 303 + 19 = 341 base pairs
Because the primer sequence are included in the PCR product at both end of the gene fragment.
Answer of Question 8
Two primers (forward primer and reverse primer) are required for amplification of polymerase chain reaction (PCR). One primer for each end of the sequence.
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