Which of the following occurs in prokaryotes but not in eukaryotes? a. post-tran

Question

Which of the following occurs in prokaryotes but not in eukaryotes? a. post-transcriptional splicing b. exchange of nucleosomes c. translation in the absence of a ribosome d. gene regulation e. concurrent transcription and translation Which of the following best describes the significance of the TATA box in eukaryotic promoters.? a. It is the recognition site for ribosomal binding. b. It sets the reading frame of the mRNA. c. It is the recognition site for a specific transcription factor. d. It defines the end of the reading frame, e. Its significance has not yet been determined The fact that plants can be cloned from somatic cells demonstrates that. a. the differentiated state is normally very unstable b. genes are lost during differentiation c. differentiated cells retain all the genes of the zygote d. differentiation does not occur in plants e. the plants have evolved in the water. In colorectal cancer, several genes must be mutated for a cell to develop into a cancer cell. Which of the following kinds of genes would you expect to be mutated? a. genes coding for enzymes that act in the colon b. genes that are not expressed in the colon c. genes that are especially susceptible to mutation d. genes of the bacteria, which are abundant in the colon e. genes invoiced in control of the cell cycle Which of the following sequences is most likely to be cut by a restriction enzyme? a. AATTCT b. AATATT c. AAAATT d. ACTACT e. ACCACT A gene that contains introns can be made shorter (but remain functional) for genetic engineering purposes by using___. a. reverse transcriptase to reconstruct the gene from its mRNA b. viruses to shorten the DNA sequence c. DNA polymerase to reconstruct the gene from its polypeptide product d. DNA ligase to put together fragments of the DNA that code foe a particular polypeptide e. a restriction enzyme (endonuclease) to cut the gene into shorter pieces

Explanation / Answer

42. e) Concurrent transcription and translation. When the mRNA will be sprouting out of the RNA polymerase, ribosomes are attached (multiple ribosomes can attach to a single mRNA to make a poly-ribosomal complex) to carry out translation, which transcription is still going on.

43. c) recognition site for a specific transcription factor. TATA box recognised by general transcription factors, which in turn are recognized by the RNA polymerase to bind to the promoter sequence.

44. c) differentiated cells retain all the genes of the zygote.

45. e) Genes involved in the control of cell cycle. As they control cell cycle, mutations in them will lead to uncontrolled cell division, leading to cancer.

46. b) AATATT. Its reverse complement is the same sequence, AATATT, which is the property of a restriction enzymes.

47. a) reverse transcriptase to reconstruct the gene from its mRNA. As an mRNA is a matured RNA, with all the introns removed, its reverse-transcripted DNA will not contain intron sequences.

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