1) DNA fingerprinting is based on the fact that: a) small amount of tissue is en

Question

1) DNA fingerprinting is based on the fact that: a) small amount of tissue is enough for analysis

b) number of repeated sequence is unique in each individual

c)DNA be isolated easily

2) The following statement is true about a proto-oncogene mutation to induce cell divission of human cell: a) proto-oncogene product must have an increased the activity

b) Proto-oncogene product must have decreased activity

c)proto-oncogene must lose ability to express mRNA

3) The following statement is true about Sanger's nucleotides sequenceing method: a) it involves in vitro DNA synthesis

b) ddNTPs terminates extension of DNA synthesis

c) it involves separation of DNA of single base length difference

d) All of the above is true

Explanation / Answer

Answer:

1). a) small amount of tissue is enough for analysis

DNA fingerprinting is a technique used to analyze DNA from human tissue samples for use in a court of law. Sources of DNA from a crime scene typically include blood, hair (with a follicle containing cells), skin and saliva (containing cheek cells). Even a small amount of tissue can yield enough DNA to generate a fingerprint. DNA fingerprinting evidence can be used to establish that a suspect committed a crime or that a suspect is free of guilt. DNA fingerprinting is based on the fact that there are regions in the human genome, which are hyper-variable between individuals.

2). c)proto-oncogene must lose ability to express mRNA

3). d) All of the above is true

Sanger sequencing is a method of DNA sequencing first commercialized by Applied Biosystems, based on the selective incorporation of chain-terminating dideoxynucleotides by DNA polymerase during in vitro DNA replication.

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