o Verizon F 12:07 PM 100% Yesterday Details 5:56 AM 10 (6 pts) Dr. Sis doing som

Question

o Verizon F 12:07 PM 100% Yesterday Details 5:56 AM 10 (6 pts) Dr. Sis doing some genetic studies on her mutty dog to try to feare out which alleles she has at the Band Eloci She performed PCR and amplified DNA sequences for both loci and is using a blue-white screening approach to cone the sequences into the vector. She cut the vector and PCR fragments with the same restriction enayme. a. What enzyme should she add to connectthe dogDNA fragments to the vector DNA 5ase. b. Dr. S then transformed the DNA into bacterial cells and selected for cells that had taken up a vector. In a single sentence, describe how she did this. e cells en latus amr allin. Dr. Snext added X-gal to her plates and screened for coloniesthat contained the vector and the insert. These colonies were Icircle one): Delete Recover

Explanation / Answer

Since this is a molecular technology, mostly Type II Restriction enzymes are used for molecular biology applications as they recognize stereotypical sequences and produce a predictable cleavage pattern.

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