True or False? 1. The genes present in a liver cell in your body willremain esse

Question

True or False?

1. The genes present in a liver cell in your body willremain essential unchanged for the life of that cell. (I think thisis false because genes can change due to mutations, etc)
2. During the SDS-PAGE portion of 2-D electrophoresis proteins areseparated by their pIs. (This is false because during SDS-PAGE,proteins are separated by mass)
3. The proteins expressed in a liver cell in your body will changesignificantly over the life of that cell. (I'm not sure...I thoughtthis was false because proteins expressed shouldn't change"significantly")
4. During the SDS-PAGE portion of 2-D electrophoresis a proteinwith 400 residues 4% of which are lys will be well separated from aprotein with 200 residues 8% of which are lys. (I don't knowhow to determine if this is true or false.)

Explanation / Answer

     1.True             Your assumption is right butthat is the case of the diseased state which we should notconsider.      We should take thegeneral things into consideration.       In general the liver cells inthe body remain unchanged in the life cycle but these(mutations)are extreme conditions       where there will bechange.       If there is anycondition provided like the cell is subjected to mutations etc.,then the answer will be false.              2.False.       In 2-Delectrphoresis the proteins are separated basing on theirmass/charge ratio and not through       their  pI's butas SDS is used the charge of the protein becomes negative and theproteins are separated basing       on their mass.       Proteins are separatedbasing on their pI's by Isoelectric electric focussing.       3.False.       As the genes are the samein the liver from the birth to the death of an organism theproteins expressed in the liver       cell in the body will notchange significantly over the life of that cell.       4.True.       In SDS-PAGE proteins areseparated on their charge by mass ratio, but as SDS is used all theproteins attain net       negative charge and theyare separated basing on their mass.       In SDS the differentsubunits of the protein are also separated.       So the subunit having8%lysine has high molecular weight than the subunit having 4%lysine so these two subunits       will be separatedseparately.        

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