5 ml of 0.05 mg/ml of ampr plasmid was used to transform bacteria in a total vol

Question

5 ml of 0.05 mg/ml of ampr plasmid was used to transform bacteria in a total volume of 50 ml. 5 ml was spread on an amp plate 100 colonies grew on the plate Calculate the transformation efficiency. It's supposed to be a very easy question the only part that I don't understand is the second step "Total used" Total mass of DNA = 0.05 mg/ml x 5 ml = 0.25 mg Total used = 1/10 of original = 1/10 x 0.25mg = 0.025 mg # colonies per mg = 100 per 0.025 mg = 100/0.025 colonies per 1 mg = 4000 = 4x103 (always expressed in Scientific Notation)

Explanation / Answer

Total mass of DNA = 0.05 mg/ml x 5 ml = 0.25 mg Total used = 1/10 of original = 1/10 x 0.25mg = 0.025 mg # colonies per mg = 100 per 0.025 mg = 100/0.025 colonies per 1 mg = 4000 = 4x103 (always expressed in Scientific Notation) +rep

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