The effect of various metal ions on alkaline phosphatase (AP) activity. Each met

Question

The effect of various metal ions on alkaline phosphatase (AP) activity. Each metal ion was added at 10 mM concentration, except zinc, which was added at 0.5 mM. Enzyme Metal Ion Added Specific Activity Native AP None 74 Metal-free AP None 4 Metal-free AP Mg2+ 45 Metal-free AP Zn2+ 29 Metal-free AP Co2+ 638 Metal-free AP Ca 2+ 3 Metal-free AP Mg2+, Zn2+ 57 6. Explain why the researchers added EDTA to the solution. Use additional references to find specific information about which metals interact with EDTA. 7. In the metal ion experiment, which condition could be considered a positive control and which condition could be considered a negative control? Which are the experimental conditions?

Explanation / Answer

The effect of various metal ions on alkaline phosphatase (AP) activity. Each metal ion was added at 10 mM concentration, except zinc, which was added at 0.5 mM. Enzyme / Metal Ion Added / Specific Activity Native AP /None / 74 Metal-free AP /None / 4 Metal-free AP /Mg2+ / 45 Metal-free AP /Zn2+ / 29 Metal-free AP /Co2+ / 638 Metal-free AP /Ca 2+ / 3 Metal-free AP /Mg2+, Zn2+ / 57

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