I generate cDNA libraries for the same cell line under different conditions. Whe

Question

I generate cDNA libraries for the same cell line under different conditions. When I compare the libraries, I find some cDNAs in one library that are missing from the other. What is the best explanation for this result? a. There was an error in generating one of the cDNA libraries and some of the sequences were not cloned properly. b. One of the cDNA libraries is really a genomic library. c. The probe I used in the Southern blot of the libraries must have been poorly constructed. d. Even the same cell type will change gene expression as conditions change. The difference in cDNAs tells me which genes are expressed in each set of conditions. e. There is no explanation other than experimental error.

Explanation / Answer

c. The probe I used in the Southern blot of the libraries must have been poorly constructed.

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