ASSIGNMENT : This is the only information given: For this exercise, you will hav

Question

ASSIGNMENT: This is the only information given: For this exercise, you will have to DO THE WORK and FIND THE ANSWER using notes. All that will be provided is the L-B plot!

1) Identify which line corresponds to the uninhibited and inhibited reaction in both hyperbolic and L_B plot.  

2)You will determine the KM and VMAX of the uninhibited reaction, and the apparent KM and  apparent VMAX of the inhibited enzyme. (DO NOT FORGET THE UNITS!)

3) Write a mechanism for each reaction and tell why the KM and Vmax change on the basis of the mechanism.

Explanation / Answer

1) In the hyperbolic (Michaelis Menten) plot, the black line represents uninhibited and the blue line represents the inhibited reaction. In Lineweaver Burks plot, the straight line represents the uninhibited and the dotted line represents the inhibited reaction.

2) For uninbited reaction:

1/Vmax = 0.75, Vmax = 1.33 umol/sec

-1/Km = -1; Km = 1 umol

For inhibited reaction:

Vmax = 1.33 umol/sec

-1/Km = -0.5; Km = 2 umol

3) This is a competitive inhibition. Both the substrates inhibitor compete for the active site on enzyme. So, Vmax remains same and Km is increased.

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