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please explain your answer! a biochemistry question (AS) The sequence below is t

ID: 282561 • Letter: P

Question

please explain your answer!
a biochemistry question

(AS) The sequence below is the amino acid sequence for the peptide drue Cys-Tyr-Ile-Gin-Asn-Cys-Pro-Leu-GlyNH2 This peptide would be not be degraded by trypsn (a protease that cleaves peptide bond foll APH owing positively charged amino acids such as Lys or Are) at neutal This peptide would be sensitve to treatment with seducing agents such as beta-mercaptoehtanol This peptide is probably not as senstive to changes in pH as it would be if it contained more amino acids with ionizable side chains All of the above are true.

Explanation / Answer

Trypsin acts on the substrate only if it contains the amino acids lysine or arginine at neutral pH. Oxytocin does not contain these aminoacids and therefore trypsin cannot act on it. But, it will be acted upon by beta-mercaptoethanol. Further, oxytocin is not as sensitive to changes in pH. So, all the options given here about oxytocin seems to be correct. Therefore, the answer for this question is option D.