UT EID: 5. What would happen if you forget to add dideoxyC (ddC) to the sequenci
ID: 282436 • Letter: U
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UT EID: 5. What would happen if you forget to add dideoxyC (ddC) to the sequencig A. The sequencing output would be the same as the one above B. The sequence would not contain any C peaks a reaction? C. The e sequence would contain C peaks at locations where they should not be tound he sequence would consist of all C peaks 6. You compare the brain cDNA library with the heart cONA ibrary. The brain cDNA ibrary has doner that contain Gene A, which none of the heart cDNA übrary clones have. The hearnt cDNA orary clones that contain Gene C, which none of the brain cDNA ibrary cones have. Both Wbrar clones that contain Gene B, but the brain library has 10 times more of such clones than the does. Which of the following is false? A.Gene A is primarily transcribed in the brain. Gene C is primarily transcribed in the heart. Gene B is transcribed in both tissue types. Gene B is expressed 10 times more in the brain than it is in the heart. The difference in the cDNA clones represented is due to some genes present in one tissue type but absent in the other. 7. What is/are correct about the whole genome sequencing? A. The genomic DNA from a single cell is sufficient for genome sequencing. B. The genome must be digested partially to generate overlapping fragments C. The overlapping fragments can be aligned to produce contigs (tretches of contiguous sequences) Both B and C E. A, Band C 8. What is true regarding SNP (single nucleotide polymorphism) and SSR (simple sequence They can both be detected through sequencing of the PCR product that contains D. An individual can have two same alleles or two different alleles for a polymorphi A. Most of them do not influence phenotypes. B. SNP is more commonly found than SSR in the genome. polymorphism locus E. All of the above TIGGGCAATAATGTAG TAACCCGTTATTACAT 9-10. Shown below is part of the last protein-coding exon of the CFTR gene. CGAG. ... ICCTAGATCTGAATTTTCExplanation / Answer
5. Sanger’s method of sequencing is also known as chain termination method. In this technique, Dideoxy nucleotides (dd NTPs)- including dd ATP, dd TTP, dd GTP and dd CTP, labelled with colored dye are added, along with d NTPS, DNA polymerase, DNA template and primers.
When dd NTPS are incorporated, there is no available hydroxyl group, for DNA polymerase, hence chain termination occurs at that point.
The reaction is carried out in several cycles, and the incorporated dd NTPS, labelled with different dyes, give different peaks in heights.
If dd CTPs are not added, Peaks for ddC will be missing.
Answer: C.
6. cDNA library contains complementary sequences to mRNA, representing almost all are most mRNA of a specific tissue. The fully transcribed mRNA from a nucleus, of the whole organism or tissue specific expression. The genes are present in all the tissues but expressed differentially.
Answer: E.
7. Whole genome sequencing (WGS), also called complete or full genome sequencing, or shot gun cloning. It involves sequencing of complete gene sequences, including that of chromosomal, mitochondrial or chloroplast (in plants) DNA.
A single cell is sufficient to contribute DNA for the whole genome sequencing.
Multiple copies of DNA are made, the restriction fragments are obtained, overlapping DNA sequence or contiguous clones (contigs) are used to sequence the entire genome.
Answer: E.
8. SNPs are Single Nucleotide Polymorphisms. SSRs are Simple Sequence Repeat. They represent phenotypic markers. They may be present in introns or exons are represented by both alleles. They are detected by PCR amplification, sequencing.
Answer:C
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