35 Which of the following is NOT present in eukaryotes? a) Aminoacyl-HRNA synthe

Question

35 Which of the following is NOT present in eukaryotes? a) Aminoacyl-HRNA synthetase Shine-Dalgarmo sequence c) Peptidyl transferase d) t-RNA 36. All the twenty amino acids have the following EXCEPT a) b) c) A hydrogen d An amino group A carboxyl group A methyl group 37. In which of the following scenarios is a silent mutation least likely? a) Change in a sequence in the 5 UTR b) Change in a sequence the 3 UTR c) Change in the sequence in the promoter Change in a sequence in an intron e) Change in a sequence in an exon 38. A bacterial sample was contaminated with an unknown preparation of vector DNA. In order to identify the vector, the bacteria was streaked on a plate and incubated overnight at 37 OC. Examination of the plate revealed at least 120 clear plaques. Which of the following is a a) The vector could be a plasmid byThe vector could be a phage c) d) The vector is a Ti plasmid The vector is pUC 39. A disadvantage of using a prokaryotic expression system for eukaryotic proteins is that the proteins are highly phosphorylated after translation b) improperly folded c) highly soluble d) Over-expressed 40. Which of the following could alter the specificity of binding of a repressor with an operator? nucleotide deletion at the operator B nucleotide addition at the operator site directed mutagenesis at the operator nucleotide deletion at the operator and nucleotide addition at the operator only nucleotide deletion at the operator, nucleotide addition at the operator, and site directed mutagenesis at the operator D. E.

Explanation / Answer

35)- option b is correct because shine dalgarno sequence present in procaryotes.

36)- option d is correct because in amino acid amino group, hydrogen group and carboxyl group present.

37)- option d is correct because in silent mutation there is no effect on protein sequence.

39)- option a is correct because protein is highly phosphorylated .

40)- option b is correct because specificity alter by addition of nucleotide at operator.

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