ANSWER ALL QUESTIONS Questions 5-6 are based on the information below A linear D

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ANSWER ALL QUESTIONS

Questions 5-6 are based on the information below A linear DNA fragment is digested with three different restriction enzymes separately - BamH I, EcoR I, and Hind III. The gel image below shows the digested product. The lanes are labeled with the enzyme that each sample was digested with. Marker Bam EcORI Hind IlI kb 13 12 10 5. This linear DNA fragment has amH I site(s), and EcoR I site(s) a. 3; 2 b. 2;1 6. The size of this linear DNA fragment is kb a. 2 kb b. 3 kb c. 5 klb d. 7 kb e. 13 kb f. 15 kb

Explanation / Answer

5. The given DNA Fragment is linear. So, if it is having two restriction sites for a restriction enzyme, it can be cut into 3 fragments. If it is having only one restriction site, then it can be cut into 2 fragments.

Hence the linear DNA Fragment has 2 BamH I sites and 1 EcoR I site.

6.The digest by BamH I has given 3 different fragments of 7, 5 and 3 kb.

The digest by EcoR I has given two different fragments of 8 and 7 kb.

The digest by Hind III has given 2 fragments of 13 and 2 kb.

Thus, the total length or size of this linear fragment is 15 kb.

Hence correct answer is "option f".

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