A phage sample has a PFU ml of 1.66 x 108 Assuming a 1:10 dilution series and pl

Question

A phage sample has a PFU ml of 1.66 x 108

Assuming a 1:10 dilution series and plating as we did in class which plate should be countable plate

(c) A phage sample has a PFU/ml of 1.66 x 10 we did in class, which plate should be the countable plate? In other words, which nlate to yield 166 plaques? Assuming a 1:10 dilution series and plati (d) You have used a phage titer procedure to determine the titer of a phage sample PFU/ml. You now want to use this phage sample to conduct an experiment, and you ne make sure each trial of the experiment uses the same phage concentration of hage Describe and/or diagram how you will use the original phage sample to prepare a dil with the desired PFU/ml for your experiment. In your descrip exactly how much phage solution and tion / diagram, you M how much sterile broth you need

Explanation / Answer

Answer: 1:1000000 dilution
Explanation:
PFU of the phage sample = 1.66 X 10^8/mL
PFU of 1:10 dilution = 1.66 X 10^7
PFU of 1:100 dilution = 1.66 X 10^6
PFU of 1:1000 dilution = 1.66 X 10^5
PFU of 1:10000 dilution = 1.66 X 10^4
PFU of 1:100000 dilution = 1.66 X 10^3
PFU of 1:1000000 dilution = 1.66 X 10^2 = 166 PFU/mL

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