I need help. are my answers to these questions correct? QUESTION 1 How does prop

Question

I need help. are my answers to these questions correct?

QUESTION 1 How does propidium iodide measure the cell cycle? Its fluorescence is proportional to the amount of DNA It's fluorescence is proportional to the amount of protein O rs tracks te cell cycle protein expression O its fuorescence is proportional to the amount of ipid QUESTION 2 Why do you have to get rid of RNA? Propidium iodide stains both DNA and RNA RNA would gum up the instrumentation O Cells won't cycle if RNA is present RNA causes cells to dye QUESTION 3 Why are the components of the staining solution dissolved in PBS rather than in water? Propid un lodide cant be di Water would cause the cells to lyse QUESTION 4 Why can't this assay distinguish between G2 and M7 O There is too much RNA at that time O They have the same proteins It is not sensitive enough They have the same amount of DNA QUESTION 5 Are the cells you are working with in this assay synchronous)

Explanation / Answer

1. Option 1 is correct.

Propidium iodide is a red-fluorescent counterstain that binds to nucleic acids (DNA and RNA). So, we can measure the amount of DNA present in a cell which is a direct indication of the cell cycle stage.

2. Option 1 is correct.

Propidium iodide is a red-fluorescent counterstain that binds to DNA and RNA.

3. Option 4 is correct.

Propidium iodide is soluble in PBS as well as in water. But, cells would be lysed due to exosmosis if we use water.

4. Option 4 is correct.

DNA replication occurs in the S-phase. So, G2 and M phase contain same amounts of DNA.

5. No

If cells exhibit different amounts of fluorescence, it shows that they are in different stages of cells cycle.

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