Observed as stated in question. AaBb -11 Aabb - 66 aaBb - 64 aabb - 15 Worksheet
ID: 281579 • Letter: O
Question
Observed as stated in question.
AaBb -11
Aabb - 66
aaBb - 64
aabb - 15
Worksheet on two and three point linkage analysis Please answer the following questions: 1. Two-point linkage analysis (test cross data) Two genes control pod length and colour in Species Q. Gene A- codes for purple colour, aa for green colour, B-codes for long pod and bb for short pod. A true breeding pure purple, long pod variety was crossed with a true breeding green, short pod variety. When the Fi plants were testcrossed with a true breeding green, short pod variety, clones with the following phenotypes were obtained: AaBb-11 Aabb 66 aaBb 64 aabb 15 a. Is there genetic linkage? (Do a chi-square test) (12 marks) b, what is the recombination %? (2 marks) c. What is the genotype of the Fi parent? (1 mark) d. What is the linkage relationship? (1 marlk) e. What is the distance between the tyo gtoes? (d mark)Explanation / Answer
Degree of freedom n-1 = 3
chi square table value at 95% = 7.81
chi square value > chi square table value 7.81
It is linked.
2. Recombination frequency = 11 + 15 / 156 X 100 = 16.66
3. AaBb
4. Genes are in same chromosome
5. 16 cM
Observed Expected O-E (O-E)2 (O-E)2/E AaBb 11 1/4X156 = 39 -28 784 20 Aabb 66 39 27 729 18.6 aaBb 64 39 25 625 16 aabb 15 39 -24 576 14.7 Total 156 69.3Related Questions
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