Normal No Spacing Heading 1 Heading To keep up-to-date with security updates, fi

Question

Normal No Spacing Heading 1 Heading To keep up-to-date with security updates, fixes, and improvements, choose Check for Updates Which of the following is common to both Sanger sequencing and next-generation sequencing? chain terminating nucleotides radioactive labels tethered DNA fragments lasers gel electrophoresis A researcher inserted DNA fragments from an organism into expression vector plasmids and introduced the modified plasmids into bacterial cells. Which of the following methods would be an effective means of identifying which clones contain a specific gene of interest? Use reverse transcriptase to prepare cDNA copies Isolate clones that still have the vector's genetic marker Assay for activity of the gene product. Prepare and analyze restriction fragments of the plasmids Sequence the DNA of the plasmids from each isolate. Michelle wants to know which cells in a particular organ produ ce a specific gene product. She has a cDNA clone of the gene available. What method would be the most useful to answer this question? microinjection of the gene fragment restriction fragment analysis real time PCR FISH (fluorescent in situ hybridization) Sanger sequencing of the clone Focus- 160 Words English (us) 1 of 1

Explanation / Answer

1. Tethered DNA fragments

2.sequence DNA plasmids from each isolate

3. Sanger sequencing of the clone

Related Questions

Navigate

• Browse (All)
• Browse N
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.