Worksheet on two and three point linkage analysis Please answer the following qu

Question

Worksheet on two and three point linkage analysis Please answer the following questions: 1. Two-point linkage analysis (test cross data) Two genes control pod length and colour in Species Q. Gene A- codes for purple colour, aa for green colour, B-codes for long pod and bb for short pod. A true breeding pure purple, long pod variety was crossed with a true breeding green, short pod variety. When the Fi plants were testcrossed with a true breeding green, short pod variety, clones with the following phenotypes were obtained: AaBb-11 Aabb 66 aaBb 64 aabb 15 a. Is there genetic linkage? (Do a chi-square test) (12 marks) b, what is the recombination %? (2 marks) c. What is the genotype of the Fi parent? (1 mark) d. What is the linkage relationship? (1 marlk) e. What is the distance between the tyo gtoes? (d mark)

Explanation / Answer

Genetic linkage by chi-square test

AABB x aabb

AaBb x aabb (Parents)

AaBb    Aabb     aaBb       aabb

What is the recombination’s:

Ans: Here recombination’s are Aabb and aaBb

What is genotype of F1 parent

Ans: The genotype of the F1 parent is AaBb x aabb.

What is the linkage relationship?

Ans: Linkage relationship is based on the crossing of two genes.

What is the distance between the two genes:

Ans: Centimorgan (cM) is the unit to measure the distance between the two genes. The distance of genes will be the one product of meiosis is 100 or one genetic map unit (m.u or cM).

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