stion 14 (10 points) D 10 points total) Xbal recognizes the sequence 5\' TGTACA

Question

stion 14 (10 points) D 10 points total) Xbal recognizes the sequence 5' TGTACA 3, and cuts between the the first T and G. In her words, Xbal will cut as follows (5' TAGTACA 3') with the carrot being the cut site. Please answer the lowing questions below based on Xbal cutting the target DNA sequence below. ' CGACACGACGCGACGTGTACATGACGACTGAC 3' (2 points) Based on where Xbal cuts with respect to the axis of symmetry, what types of overhangs would ou predict after cutting the DNA b. (2 points) How many Xbal sites are found in the DNA molecule above? After digesting the DNA above with Xbal, how many fragments will you get? c. (2 points) Please state whether the Xbal site is palindromic and why it is palindromic. d. (4 points) Please diagram out the fragments you will get after cutting with xbal

Explanation / Answer

5’ CGACACGACGCGACGT…GTACATGACGACTGAC 3’

3’ GCTGTGCTGCGCTGCACATG….TAGAGCTGAGTG 5’

B.

2SITES ARE THERE IN MOLECULE FOR Xbal and will get 4 fragment after digestion.

c. this site is has palindromic sequence because 3’ to 5’ and 5’ to 3’ sequence of restriction site of both are same when read it both site.

d. diagram of fragment

5’ CGACACGACGCGACGT…GTACATGACGACTGAC 3’

3’ GCTGTGCTGCGCTGCACATG….TAGAGCTGAGTG 5’

(….. indicate restriction site)

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