Potentially tricky questions: a. Clestridium perfringens is a soil bacterium tha
ID: 280679 • Letter: P
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Potentially tricky questions: a. Clestridium perfringens is a soil bacterium that is well known for causing gas gangrene. gangrene results in tissue necrosis and can lead to amputation of the affected limb and treated, death. C. perfringens must grow anaerobically- that is in the complete absence of ox However, C perfringens has an electron transport chain and an ATP synthase. i. think C. perfringens grows via respiration or fermentation? Why? b. Two cultures of yeast are grown side by side to the same population size of 10,000,000 growth medium for each culture is the same: an extract of malted barley which is mos glucose. Culture "A" was grown aerobically (with oxygen) and culture "B" was grown anaerobically (without oxygen). i. Which culture reached 10,000,000 cells first? (A/B /reached at same time) ii. Which culture used more glucose to get to 10,000,000 cells? (A/B/ equal) ili. Which culture produced carbon dioxide? (A/ B/both / neither) iv. Which culture produced ethanol? (A/B/both / neither) v. Which culture produced lactic acid? (A/B/ both / neither) vi. Which culture used NAD? (A/B/ both / neither) vii. Which culture produced more ATP? (A/B/ equal)Explanation / Answer
B) Ans 1) B will grow fast.
This is because yeast grow better in anareobic condition.
Ans 2) B will use more glucose
Ans 3) B will propduce more CO2
4) B will propduce ethanol.
5) B will produce lactic acid.
6) Both will iuse NAD+.
In aerobic process NAD+ will be used after glycolysis and in anerobic process NAD is used during alcohol fermentation.
7) A will produce more ATP.
In aerobic process ATP is produced by the mitochyondria which is the power house of the cell. In anaerpbic process ATP is not produced in large quantities.
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