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5. Next, assume that you redo the experiment as follows: add mitochondria to the

ID: 280653 • Letter: 5

Question

5. Next, assume that you redo the experiment as follows: add mitochondria to the clean flask, allow the reaction to proceed for one minute, then add succinate to the flask. Formulate a hypothesis to explain what you think will happen if you add succinate to the flask. Explain why the addition of succinate would produce this effect on oxygen consumption. 6. Finally, assume that you repeated the procedure above as separate experiments for each of the following substrates: fumarate, ascorbate/TMPD (tetramethyl-p-phenylene diamine), malate, and ADP. (Note: Ascorbate and TMPD are synthetic electron donor molecules that can supply electrons to the electron transport chain.) For each of these experiments, would the effect on oxygen consumption appear to be the same for each substrate? Describe any obvious differences in oxygen consumption which you would observe with the different substrates. Based on what you know about the purpose of each substrate in the reactions of cellular respiration, provide possible explanations

Explanation / Answer

ans-5 the succinate is an enzyme which is the intermediate of the krebs cycle and it play significant role in the cellular respiration, intake and uptake of various minerals and ATP synthesis.

hypothesis - the addition of the the succinate will increase the consumption of the oxygen consumption because the succinate tend to increase the cellular respiration and the production of ATP and this process require oxygen in a high level.

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