10. When you make your agarose gel, you will need to make 50 ml of 1% (w/v) agar
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10. When you make your agarose gel, you will need to make 50 ml of 1% (w/v) agarose in 1X TAE. How much agarose will you require? [you will make this later, not on Solution day] When adding your DNA samples to the agarose gel, you will need to add loading dye to the samples. Loading dye contains: glycerol, which helps the samples sink into the sample wells of the gel: and tracking dyes so that you have a way of estimating how far the samples have travelled through the gel during electrophoresis. The recipe for 6X loading dye is as follows: 0.25% bromophenol blue (w/v) 0.25% xylene cyanol (w/v) 30% glycerol in water (v/v)Explanation / Answer
1% means there should be 1grams of agarose in 100ml of TAE. So, by simple M1V1=M2V2, in 50ml, u will need to add 0.5 grams of agatose.
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