Probe 3 Probe 4 Exon 1 Intron Exon 2 Intron Exon 3Intron Exon 4 In the box above

Question

Probe 3 Probe 4 Exon 1 Intron Exon 2 Intron Exon 3Intron Exon 4 In the box above is a section of chromosomal DNA showing YFG with exons and introns highlighted. Also shown on that diagram are 4 different northern blot probes. Use the answer choices in the box below to answer the following questions. Some answer choices may be used more than once. Other answer choices might not be used at all. What will Probe 1 reveal? What will Probe 2 reveal? What will Probe 3 reveal? What will Probe 4 reveal? A. B. C. D. Answer Choices Pre-mRNA ONLY mRNA ONLY Both Pre-mRNA and mRNA Nothing, it is a useless probe

Explanation / Answer

To answer the question, we need to clarify the answer choices.

Pre-mRNA only: Pre-mRNA contains both exons and introns. Introns are removed to produce mRNA. So the probe must be specific for intron to only to be detected in pre-mRNA.

mRNA only: present Only in exon but not functional in pre-mRNA. Because after splicing (removal of introns) non-functional probes are joined together to form a functional probe.

Both pre-mRNA and mRNA: Present in exon and functional.

Nothing, it is a useless probe: Present in introns and Not functional.

Probe 1: Both pre-mRNA and mRNA

Probe 2: Pre-mRNA only

Probe 3: Nothing, it is a useless probe

Probe 4: mRNA only

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