Question\'7 10 marks 10 minutes Genetic analysis was used to dissect the pathway
ID: 280041 • Letter: Q
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Question'7 10 marks 10 minutes Genetic analysis was used to dissect the pathway for biosynthesis of methionine in a haploid fungus species. The pathway was determined to be met-5+ met-3+ met-2+ met-8+ homoserineacetyl homoserine cystathioninehomocysteinethionine Copy the table below into your answer book to reproduce the experimental results that led to determining this pathway. Fill in "growth" or "no growth" as appropriate. [10 marks] Minimal growth medium supplemented with: crystathionine acetyl homoserine homoserinehomocysteine methionine met-2 mutant met-3 mutant met-5 mutant met-8 mutantExplanation / Answer
A
Cystathione
B
Acetyl homeserine
C
Homoserine
D
Homocysteine
E
Methionine
Met-2 mutant
Growth
Growth
Growth
No Growth
No Growth
Met-3 mutant
No Growth
Growth
Growth
No Growth
No Growth
Met-5 mutant
No Growth
No Growth
Growth
No Growth
No Growth
Met-8 mutant
Growth
Growth
Growth
Growth
No Growth
The methionine is the end product and cannot revert to the reactants. So the mutants that require homoserine(Met-5), Acyhomoserine(met-3), cystathionine (met-2), and homocysteine(met-8) will not be able to gwo on media supplemented with methionine.
While the media supplemented with homoserine will be favorable for growth of all the mutants as the pathway will lead to formation of other products in series from reactant to product.
A
Cystathione
B
Acetyl homeserine
C
Homoserine
D
Homocysteine
E
Methionine
Met-2 mutant
Growth
Growth
Growth
No Growth
No Growth
Met-3 mutant
No Growth
Growth
Growth
No Growth
No Growth
Met-5 mutant
No Growth
No Growth
Growth
No Growth
No Growth
Met-8 mutant
Growth
Growth
Growth
Growth
No Growth
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