PAGE 201 ) Name (Last, First): Il. Use the DNA sequence below, which encodes a p
ID: 279726 • Letter: P
Question
PAGE 201 ) Name (Last, First): Il. Use the DNA sequence below, which encodes a prokaryotic gene to answer the following questions. 21 71 121 31 81 131 41 ATGAGGAGTT GACACACAAG AGGAGGTAGC AGTATGGGTA TAATCTAATG 51 CGTAATTGAG GAGGTAGTTG ACGTATGAAT AGTTAACGTA CGGGGGGGAA 101 ACCCCCCCTT TTTTTTTTTC GAGCAATAAA AGGGTTACAG ATTGCATGCT 61 91 141 a) Write down the corresponding sequences, find them in the sequence above and label them: (label as -35) 35 Consensus sequence: -10 Consensus sequence (Pribnow box): Shine-Dalgarno sequence in corresponding mRNA: Start (initiation) codon in corresponding mRNA: Stop (termination) codon in corresponding mRNA: (label as Pribnow) (label as SD) (label as start) (label as stop) b) Whatregion of this prokaryotic DNA sequence will be transcribed into mRNA? Circle one. 1-131 71-119 74-149 54-119 a) What will the sequence be for the protein translated from this mRNA? c) Where is the 3'UTR? Circle one. 9-131 41-119 81-141 87-119Explanation / Answer
3) A) -35 sequence is TTGACA (9-14)
Pibnow box is TATAAT (39-44)
Shine- dalgarno sequence is AGGAGG (59-64)
start codon (75-77)
stop codon TTA (84-86)
B) 54-119
C) met-asn-ser
D) 87-119
IV) A) TATAAA (5-10)
Poly A signla AAUAAA ( 75-80)
Kozaks sequence CCAUGG ( 38-43)
Start codon ATG (51-53)
Stop codon TAA( 59-62)
ORF (51-59)
UTR (63-101)
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