NEED TO FILL IN CHART ONLY... Thanks Tet Bor She Picture T Step 1: The following

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NEED TO FILL IN CHART ONLY... Thanks

Tet Bor She Picture T Step 1: The following table shows a partial DNA, mRNA, tRNA and amino acid sequence for part of a gene associated with a disease. Answer the following questions to gain insight on how to complete the sequences. Fill in the table below; include the 5' and 3' ends of each DNA/RNA sequence and the C terminal and N terminal ends for the amino acid sequences. Once the table is complete and you have obtained the single letter amino acid sequence, continue to step 2. Tip: you may want to use a pencil. DNA Double Helx mRNA U G GIU THR tRNA anticodon Amino Acid Single Letter AA MET GLU VAL CYS 1.) mRNA may be transcribed from either strand of the DNA molecule. Therefore, it is important to determine which strand is the coding strand and which strand is the template strand. From the information in the table, are you able to identify which strand is which? How? 2.) What will be the only difference between the DNA coding strand sequence and the mRNA scquence? 3.) What will be the only difference between the DNA template sequence and the tRNA anticodon sequence? 4.) Which direction will our mRNA molecule be TRANSLATED: left to right or right to left? How do you know? 138% Sec 1Pages:1 of 2 Words:96 of 381

Explanation / Answer

5’ ATG CTC ACA TTC ATG GCC TCT GAC AGC GAG GAA GAA GTG TGT GAT Coding strand

3’ TAC GAG TGT AAG TAC CGG AGA CTG TCG CTC CTT CTT CAC ACA CTA Template strand

5’ AUG CUC ACA UUC AUG GCC UCU GAC AGC GAG GAA GAA GUG UGU GAU mRNA strand           

3’ UAC GAG UGU AAG UAC CGG AGA CUG UCG CUC CUU CUU CAC ACA CUA tRNA strand

N-Terminus MET-LEU-THR-PHE-MET-ALA-SER-ASP-SER-GLU-GLU-GLU-VAL-CYS-ASP C-terminus Amino Acid

N-terminus M-L-T-F-M-A-S-D-S-E-E-E-V-C-D c-terminus Single Letter AA

1. the 5’ to 3’ strand is of coding strand and the 3’ to 5’ strand is of template strand

2. the difference between the DNA coding and mRNA anticodon sequence is that the mRNA has uracil instead of thymine in the DNA template stand

3. the difference between the DNA template and tRNA anticodon sequence is that the tRNA has uracil instead of thymine in the DNA template stand

4. The mRNA molecule will br translated in the direction from LEFT to RIGHT

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