16. (2 pts). You have growth on all three plats (MAC, BAP, CNA). You decide to p
ID: 279549 • Letter: 1
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16. (2 pts). You have growth on all three plats (MAC, BAP, CNA). You decide to pick the bright pink colonies off of your MAC and get the following results. KIA is yellow/yellow with no blackening There is some red on your slant though but glucose and lactose phenol red broths are both yellow. Your Indole test produces no red color, Methyl Red test does not turn red, but your v test does. Citrate is a dark cobalt blue. Urea has stayed a salmon color and your oxidase test does not turn blue. VP You turn your attention to your CNA plate and pick from it. The colonies have a greenish color in the blood surrounding them. The catalase test does not bubble, you do a spread plate with an Optochin disk and find a large area of no growth after incubation What two bacteria do you have? 17. (3 pts). Describe to me what a positive reaction and a negative reaction for each of the following would look like. a. Citrate b. 6.5 % salt broth c. Hydrogen Sulfide (H2S) production Bonus 1(1 pt). What broth do you use to do the methyl red test? Bonus 2 (1 pt). What broth do you use to do the Indole test?Explanation / Answer
16) The bright pink colonies on MAC agar are produced by gram negative bacteria. Mostly, the gram negative bacteria include enteric pathogens like Escherichia coli, Klebsiella and Enterobacter. The bright pink colonies from MAC agar tested positive for VP (Voges-Proseuker) test. Klebsiella tests positive in VP test. So, this bacteria is most likely to be klebsiella pneumoniae.
Columbia nalidixic acid (CNA) agar is a selective medium for gram positive bacteria. The bacteria is catalase negative and optochin sensitive. So, the most likely bacteria is Streptococcus pneumoniae.
So, the two bacteria are,
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