For each question, circle the letter of the correct answer. Note that some quest
ID: 279518 • Letter: F
Question
For each question, circle the letter of the correct answer. Note that some questions have more than one answer. For these questions, provide all the correct answers. Each correct answer is worth 0.5 point for a possible total of 10 extra credit points. I expect you to work on this assignment independently. If it becomes clear that students collaborated on this assignment, then no extra credit will be awarded. A molecule of the motor protein Winnebago, supplied with ATP, is moving along a microtubule in the direction shown in the figure below. What will happen if you suddenly remove all ATP from the system by adding an enzyme that hydrolyzes ATP? 1. A. No change: Winnebago will continue to move from A to B. B. Winnebago will wander back and forth along the microtubule. C. Winnebago will move backwards (towards point A instead of point B) D. Winnebago will stall on the microtubule. E. Winnebago will continue to move from point A to point B, but at a slower rate A poison added to an in vitro translation mixture containing mRNA molecules with the sequence 5'- AUGAAAAAAAAAAAAAAAUAA-3' has the following effect: the only product made is a Met-Lys peptide that remains attached to the ribosome. What is the most likely way in which the poison acts to inhibit protein synthesis? 2. A. It inhibits binding of the small subunit of the ribosome to mRNA. B. It inhibits peptidyl transferase activity C. It inhibits translocation of the ribosome. D. It inhibits release factor. E. It mimics release factor. After isolating the rough endoplasmic reticulum from the rest of the cytoplasm, you purify the RNAs attached to it. What proteins do you expect these RNAs to encode? 3. A. B. C. D. E. soluble secreted proteins ER membrane proteins mitochondrial membrane proteins plasma membrane proteins ribosomal proteins Questions 4 to 6. Complete each of the folowing sentences correctly by circling the correct response from each pair. New plasma membrane reaches the plasma membrane by the [regulated / constit pathway utive] exocytosis 4. / constitutive] exocytosis 5. New plasma membrane proteins reach the plasma membrane by the [regulated/constitutive] exocytosis 6. Insulin is secreted from pancreatic cells by the [regulated/ constitutive] exocytosis pathwayExplanation / Answer
1. D. Winnebago will stall on the microtubule. Motor proteins that have unidirectional movement requires constant input of energy from ATP hydrolysis, for their movement. If there is no ATP, there is no force to drive them, they stall immediately.
2. C. It inhibits translocation of the ribosome.
3. A, B, and D. Except mitochondrial membrane proteins and ribosomal proteins, all other proteins are expected to be synthesized.
4. Constitutive
5. Constitutive
6. REgulated
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