ndvDocuments exam%20prep%201 st%20sem/wtw%20154%20prep Examis2020 13%20for%20dic
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ndvDocuments exam%20prep%201 st%20sem/wtw%20154%20prep Examis2020 13%20for%20dickUp%20%201).pd 8.3 The amino acid sequences in the table below were obtained fom the central region of a particular polypeptide chain in a wild-type and a inutant bockerial strain. The mutation arose from a change at the DNA level involving a single nucleotide./ Die aminosuur volgordes in die onderstaande tabel is verkry vanvan die sentrale gebied van 'n sekere polipeptied ketting in 'n wildetipe en 'n mutante bokteriele stam. Die mutasie het uit 'n verandering op DNA vlak onstoan woar 'n enkel nukleotied betrokke was. a. Provide the correct mRNA codons from table 2 for the amino acids CYs and SER in the wildtype polypeptide and LEU and STOP in the mutant polypetide that make this scenario possible. Verskaf die korrekte mRNA kodons uit tabel 2 vir die aminosure CYS en SER in die wildetipe polipeptied en LEU en STOP in die mutante polipeptied wat hierdie situasie moontlik maak. (4x%) Codons and amino acids/ Kodons en aminosure Wild type Codons / Wildetipe Kodons AUG GCG ALA Amino acidsMET CYS SER Mutant /Codons/ Kodons AUG GCG ALA Amino acids MET Aminosure LEU STOP Explain how this mutation arose [1 mark]. (In your answer include: the name of the type of mutation [1 mark]: and fully explain the consequences 13 marks]). Verduidelik hoe hierdie mutosie onststaan het [1 punt). (In u antwoord sluit in: die noam van die sort mutosie [1 punt), en verduidelik die gevolge volledig [3 punte)]) b. total 5) / totoal 5)Explanation / Answer
Answering 3 questions based on CHEGG rules:
8.3a. WIld type: UGU/UGC corresponds to CYS and for SER it will be UCU/UCC/UCA/UCG
mutant : LEU CUU/CUC/CUA/CUG and stop codon: UAG/UGA/UAA
b. The mutation may have cause due to environmental factors like UV radiation, or during the time of DNA copying and proofreading activity of DNA polymerase.
8.2 a. For question 8.2, if one has the sequence use BLASTN to identify the sequence, look at the nucleotides that are conserved across many species.
bFor obtaining the frames, one can use Expasy, a free online protein translate tool which gove protein translation in both 5' to 3' as well as 3' to 5' translation including the stop codons if present
7.4 The process is protein synthesis (mRNA being translated into a polypeptide).
b. The process occurs in prokaryotes as well as eukaryotes as the fundamental principle of molecular biology remains the same in both organisms. DNA-RNA-polypeptide-protein is common for both pro and eukaryotes but in case of eukaryotes the events are complex and it occurs in a compartmentalized form.
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