Practice Exam 3-1 Name ns between four proteins, w, X. Y, and Z. You created the

Question

Practice Exam 3-1 Name ns between four proteins, w, X. Y, and Z. You created the two reporae u nstructs below in order to complete your analysis. You engineered a strain of yeast that contains both of these constructs site site Additionally, you made the following chimeric proteins: W with the the lexA DN GALA DNA binding domain, X with A binding domain, Y with the lexA activation domain, and Z with the GALA activation domain W GAL4 DBD x lexA DBD Y lexA AD Z GAL4 AD Addition of any single chimeric protein to your yeast strain does not lead to expression of GFP or lacZ. To understand the interactions between the proteins, you introduce them into your yeast cells in a pairwise manner. Which of the following statements is correct? a. If X interacted with W and Z, you would observe expression of lacZ, but not GFP. b. If W interacted with Y, you would not observe expression of GFP or lacz. c. If Z interacted with X, you would observe expression of GFP, but not lacZ. d. If Y interacted with Z, you would observe expression of both GFP and lacz. e. If X interacted with Y and Z, you would observe expression of both lacZ and GFP

Explanation / Answer

Bait= W- GAL4 DBD and X- lexA DBD

Prey= Y- lexA AD and Z- GAL4 AD

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