clear image of graphs Here is graph of describing what happened to a culture whe

Question

clear image of graphs

Here is graph of describing what happened to a culture when it was exposed to a temperature higher than it's maximum temperature 1. The following tow questions are about using heat to kill microorganisms. a. What is the decimal reduction time cFU/m for this organism? (1 point) 100 004500 0 Time in seconds b. You repeat DRT experiment with a Gram-positive rod and this is the result. Your water bath maintained a constant temperature; your dilution and plating technique is perfect. All the colony morphologies match that ove your original culture. What would cause this result? (1 point) CFU/ml 0 100 20 30000 00 600 7100 Time in Seconds Lacl and LacZ are proteins encoded by genes, and there can be mutated forms of them. The place where Lacl binds, lac0, is also a DNA element, and is subject to mutation. In the table below, I've listed a number of potential mutations. I want you to tell me after growing each mutant in a minimal medium with equal parts glucose and lactose, both sugars at 0.2% do you think you'd see blue in a ß-galactosidase assay? (2 points) 2. Blue or No Color Change? Mutation A Lacl mutated so that it no longer recognizes laco. A Lacl that no longer binds lactose A LacZ that no longer cleaves lactose A lac0 mutated so Lacl no longer recognizes it. A one base frame shift mutation after the first 9 bases of lacl

Explanation / Answer

1.A. 190 seconds
B. Formation of endospores

2. ?-galactosidase can be detected by X-gal, a colourless analog of lactose that may be cleaved by ?-galactosidase to form 5-bromo-4-chloro-indoxyl, which then spontaneously dimerizes and oxidizes to form a bright blue insoluble pigment
1. Blue -
2. No color change
3. No color change
4. Blue
5. Blue

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