Part 2 (questions 31-35, 2pts each) The following 5 questions are about the pape

Question

Part 2 (questions 31-35, 2pts each) The following 5 questions are about the paper by D et, al DiNardo, t was the name of the amino acid that the author was investiganting in the DiNardo paper? A) Leucine C) Cystiene E) Aspartate B) Proline D) Alanine 32) What is the function of the aromatase enzyme? A) It's a lyase C) It changes testosterone to estrogen (17B-estradiol) D) It phosphorylates a serine on estrogen E) None of the above 33) Why did the mutation of the D309N ruin the effect of human aromatase expressed in E.Col A) D is polar and N is non-polar C) D is polar and so is N E) None of the above B) D can be titrated and N is not titratable. D) D cannot be titrated and N can be titrated. u) When measuring the effects of ph, how did the authors prove that the ionie strength of the buffer being used wasn't leading to false data? A) They used all the same concentrations of buffers B) They used all the same bufers C) They used different buffers at each pH D E) None of the above They didn't use buffers at all 35) What reason did the author suggest for exemestane having a higher pKa than androstenedione when binding compared to androstenedione? A) The Km was different between them B) There are additional hydrophobic contacts made. C) The Kd and Ka were different between them E) None of the above D) Their Kcat were different

Explanation / Answer

31. Aspartate

32. B (last step from androstenodione to estrone and testoterone to oestradiol

33. Asp can be titrated whereas asn cannot be titrated

34. They used different buffers at eac pH

35. The Kd and Ka are different between them

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