(2)) Below are diagrams of the lac operon region in E. coli. (a) is wild-type, b
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Question
(2)) Below are diagrams of the lac operon region in E. coli. (a) is wild-type, but the others have one or more of the components (either genes or binding sites) moved to different locations, for each state if ?-galactosidase protein will be detected in the presence or absence of lactose and/or glucose (indicate yes or no in the table, as always assume very low levels to be no) no glucose no glucose with glucose with glucose with lactose no lactose with lactose no lactose ac temwierO i promoter CRP siteOperator lac promoter acAI ac CRP site (c) ,promoter CRP site terminator Operafor (d) i promoter CRP siteOperator ac terminator lacA (e) i pronoter CRP siteOperator promoter ac promoter lacA (0 i promoter Operat promoter lac./ CRP site terminafor lacl acz (g) lac promoter CRP siteOperatorExplanation / Answer
a)
Yes
No glucose with lactose No glucose no lactose With glucose with lactose With glucose no lactose Commenta)
Yes
No Yes(low level) No b) Yes No Yes( low level) No Lac repressor is a trans-acting element. c) Yes Yes Yes (low level) Yes (low level) Lac operator is a cis-acting element. d) Yes Yes Yes Yes lac I promoter is a constitutively expressed promoter. e) Yes No Yes (low level) No beta-galactosidase gene is not affected by the change in location. f) Yes(low level) Yes (low level) Yes (low level) Yes(low level) CAP interacts with C terminal domain of RNAP. The location change disrupts this interaction. g) Yes Yes Yes(low level) Yes (low level) Lac repressor will not be coded anymore, because it is under control of lac promoter.Related Questions
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