3. To study bacterial ribosome elongating prokaryotic ribosome when the followin

Question

3. To study bacterial ribosome elongating prokaryotic ribosome when the following antibiotics are present. a. Tetracydine: binds in the A site a blocks entry of amino-acyl tRNA to the A site of ribosome. s, researchers make use of antibiotics that inhibit ribosome function at different steps of present (uncharged-tRNA, amino acyl-tRNA, peptidyl-tRNA, et.c..) in the A and P sites of an be b. Kanamyocin: prevents translocation. c. Kirromycin: prevents release of EF-Tu/GDP from aa-tRNA. d. Puromycin: can bind the ribosome peptidyl transfer active site and accept the growing peptide chain. e. Ricin: cleaves the rRNA which inactivates its GAP function.

Explanation / Answer

a. Use of tetracycline would lead to an uncharged tRNA as it blocks the early step of protein synthesis.

b. Kanamycin would lead to amino acyl tRNA as the uncharged tRNA along with elongation factors move into the ribosome for incorpration into polypeptide chain.

c. One would find a peptidyl tRNA in case of use of kirromycin as it inhibits the GTPase activity of EF-Tu. All process that requires EF-Tu is affected. Peptide formation is affected at the time of incorporation of Phe-tRNAPhe to the ribosome.

d. Puromycin would lead to premature chain termination and part of the molecule resembles 3' end of aminoacylated tRNA.

e. Ricin inactivates the ribosome 60S. They are toxins that are also called ribosomal inactivating proteins. Thus entire process of protein synthesis gets severely affected.

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