-3 1 23 SsDNA template: S-ATATCGCTACGTCCAGTTTACGTCAAGTTGCACACGTGCAGA DNA polymer
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Question
-3 1 23 SsDNA template: S-ATATCGCTACGTCCAGTTTACGTCAAGTTGCACACGTGCAGA DNA polymerase, dATP, dTTP, dGTP Reaction 1: SSDNA primer: 5-TATAGCGATGCAGGTCAAATGC-3 dCTP Draw annealing of primer and direction of primer extension 5-ATATCGCTACGTCCAGTTTACGTCAAGTTGCACACGTGCAGA-3 Reaction 2: SSDNA primer: 5-TCTGCACGTGTGCAACTTGACG-3dCTP Draw annealing of primer and direction of primer extension 32 5-ATATCGCTACGTCCAGTTTACGTCAAGTTGCACACGTGCAGA-3 31 Reaction 3: SSDNA primer: 5-TCTGCACGTGTGCAACTTGACG-3 27 dcTP& ddCTP (10:1)2 Draw annealing of primer and direction of primer extension 5-ATATCGCTACGTCCAGTTTACGTCAAGTTGCACACGTGCAGA-3 2.) Retroviruses Retroviruses can insert DNA randomly into the human genome. Depending on its location this integration can have consequences of varying severity. Describe 3 general integration scenarios. (Where does the retroviral DNA integrate? What are the consequences?) (Word limit: 60) (9 points) 3.) RNA secondary structure Due to its single stranded nature RNA can basepair with itself and form secondary structures like stem loops. Describe 3 examples of RNA secondary structures and their function. (Word limit: 60) (9 points)Explanation / Answer
2. Retrovirus integrates into host cell's genome and provides stable gene expression.
3 consequences of integration
1. It contains no viral genes
2. Few immunity problems
3. Random integration may cause insertional mutations.
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