C) Use a standard curve to find the concentration of an Unknown solution A stand
ID: 277990 • Letter: C
Question
C) Use a standard curve to find the concentration of an Unknown solution A standard curve is a type of graph made using a few solutions of known concentration (standards). The curve is then used to find the concentration of an unknown solution. 0.180 0.160 E 0.140 8 0.100 0.080 0.080 0040 0.020 0.000 5 0.0 20 4.0 6.0 8.0 10.0 12.0 Concentration of BSA (g/ml) Bovine Serum Albumin (BSA) is a protein. The graph shows the relationship between concentration of BSA protein and absorbance and is known as a standard curve A spectrophotometer is used to accurately determine the amount of light absorbed by a colored solution. These absorbance values are plotted against the concentration to develop the standard curve as shown above. The concentration of an unknown solution can be Use the graph to answer these questions: extrapolated from this standard curve a. What conclusion can you draw from the data depicted in the graph? b. A BSA solution has an absorbance value of 0.100, use the graph to determine the concentration of the solution. If a BSA solution has a concentration of 8.0 ug/ml, what absorbance value can you predict it will give when placed in a spectrophotometer? c.Explanation / Answer
a. the graph here depicts a standard curve for BSA. Serially diluted BSA solution has been used tand absorbance values are recorded to generate this standard curve.
b. if the BSA solution has the absorbance of 0.100, the concentration of the solution will be around 6.2 ?g/ml.
c. if the BSA solution has the concentration 8?g/ml, the absorbance will be around 0.120
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