Rat liver mitochondria were added to a lightly buffered medium that contains ADP

Question

Rat liver mitochondria were added to a lightly buffered medium that contains ADP, rotenone, and antimycin A. Ferrocyanide which donates electrons to cytochrome c was then added to initiate the experiment. What conclusions can you draw from the experimental results that are depicted below? Using your knowledge of electron transport and chemiosmotic systems answer the following questions relating to these experimental results.

a.)What is the basis for the increase in H+ concentration in the portion of the plot labeled as “a” and the subsequent decrease, labeled as “b”? Why does the H+ concentration initially increase rapidly, and then after a period of time, slowly decrease?

b.) Why does the [H+] decrease rapidly when an uncoupler such as FCCP is added?

c.) How would the addition of atractyloside at the beginning of each of this experiment affect the results? Sketch a plot to illustrate the experimental results. What is the biochemical basis for this effect?

Explanation / Answer

a)

b)

c)

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